Kate Walton

How Do Primers and Probes Affect Sequence Detection Results? Primers and probes can make the difference between a clean sequence detection result and a confusing one. In real-time PCR or qPCR, primers decide what DNA or RNA target gets copied. Probes, when used, decide what signal gets reported. When they are designed well, the system […]

How Can Contamination Affect a Sequence Detection System Result? A sequence detection system is built to find tiny amounts of DNA or RNA. That sensitivity is what makes qPCR, RT-qPCR, and other nucleic acid tests so useful in disease testing, research, food testing, and environmental monitoring. The same sensitivity also makes contamination a serious source

Can Sequence Detection Systems Identify Genetic Mutations? Sequence detection systems can identify genetic mutations, but the level of detail depends on the method being used. A real-time PCR system can detect a known mutation when the test is designed for that exact change. A sequencing-based system can read larger parts of DNA or RNA and

The Difference Between Applied Biosystems 7500 and Other Sequence Detection Systems The Applied Biosystems 7500 Real-Time PCR System is a 96-well sequence detection system built for real-time PCR, gene expression, SNP genotyping, pathogen detection, and other nucleic acid quantification work. Its main difference from many newer sequence detection systems is that it is a trusted,

How Do You Choose the Right Sequence Detection System for a Lab? Choosing the right sequence detection system starts with one honest question: what decision will this result help your lab make? A small research lab tracking gene expression does not need the same setup as a hospital lab running infectious disease assays, a cancer