What Is a No-Template Control in Sequence Detection Testing? A no-template control in sequence detection testing is a blank test reaction that contains every testing ingredient except the DNA or RNA sample being checked. Its job is simple: prove that a positive signal came from the real sample, not from contaminated reagents, carryover DNA, primer-dimers,
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Why Do Some qPCR Amplification Curves Look Abnormal? Some qPCR amplification curves look abnormal because the reaction is no longer behaving like a clean, target-specific PCR. The cause may be simple, such as a bad baseline setting, pipetting variation, low template amount, or a weak fluorescent signal. It may also point to a deeper problem,
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How Do Primers and Probes Affect Sequence Detection Results? Primers and probes can make the difference between a clean sequence detection result and a confusing one. In real-time PCR or qPCR, primers decide what DNA or RNA target gets copied. Probes, when used, decide what signal gets reported. When they are designed well, the system
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How Can Contamination Affect a Sequence Detection System Result? A sequence detection system is built to find tiny amounts of DNA or RNA. That sensitivity is what makes qPCR, RT-qPCR, and other nucleic acid tests so useful in disease testing, research, food testing, and environmental monitoring. The same sensitivity also makes contamination a serious source
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Can Sequence Detection Systems Identify Genetic Mutations? Sequence detection systems can identify genetic mutations, but the level of detail depends on the method being used. A real-time PCR system can detect a known mutation when the test is designed for that exact change. A sequencing-based system can read larger parts of DNA or RNA and
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The Difference Between Applied Biosystems 7500 and Other Sequence Detection Systems The Applied Biosystems 7500 Real-Time PCR System is a 96-well sequence detection system built for real-time PCR, gene expression, SNP genotyping, pathogen detection, and other nucleic acid quantification work. Its main difference from many newer sequence detection systems is that it is a trusted,
How Do You Choose the Right Sequence Detection System for a Lab? Choosing the right sequence detection system starts with one honest question: what decision will this result help your lab make? A small research lab tracking gene expression does not need the same setup as a hospital lab running infectious disease assays, a cancer
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